Glass Bioreactor
(Laboratory Benchtop Bioreactor, Off-site Sterilization)
Sterile. Scalable. Smart. (500mL - 10L)
The laboratory glass fermenter is a small-scale bioreactor designed specifically for research in bio-engineering, pharmaceuticals, food, and environmental science. It is primarily used for cultivating microorganisms in a controlled environment. The high-transparency borosilicate glass vessel allows for easy observation of growth status and contamination anomalies. It is corrosion-resistant, easy to clean, and simple to install.
The FZ-B series laboratory benchtop bioreactor is engineered to obtain stable, repeatable fermentation data within a small volume range of 0.5L to 10L, providing a critical reference for subsequent pilot-scale or stainless steel system scale-up. It is precise and flexible, suitable for diverse scientific research and preparation for industrial-scale production.
- Capacity: 0.5L / 1L / 3L / 5L / 10L (customizable)
- Vessel Material: High borosilicate glass
- Lid & Base Material: SUS316L stainless steel
- Number of Vessels: 1 to 4 units optional
- Working Volume: 30% - 75%
- pH: 0-12 (±0.01)
- DO (Dissolved Oxygen): 0-200% (±0.1)
| Model | ||||
| Control Functions | Temperature | Measurement & Control | Measurement & Control | Measurement & Control |
| pH | Measurement & Control | Measurement & Control | Measurement & Control | |
| DO | Measurement & Control | Measurement & Control | Measurement & Control | |
| Agitation Speed | Measurement & Control | Measurement & Control | Measurement & Control | |
| Sterilization | Measurement & Control | Measurement & Control | Measurement & Control | |
| Pressure | Measurement & Control | Measurement & Control | Manual | |
| Flow Rate | Measurement & Control | Manual | Manual | |
| Acid/Alkali | Automatic Control | Automatic Control | Automatic Control | |
| Foam Control | Automatic Control | Automatic Control | Automatic Control | |
| Feeding | Peristaltic Pump | Peristaltic Pump | Peristaltic Pump | |
| Applicable Use | Suitable for process development and pre-scale-up studies requiring high-level fermentation process control. | Suitable for process development and pre-scale-up studies requiring high-level fermentation process control. | Suitable for teaching experiments and basic fermentation research with lower automation requirements. | |
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Vessel
- Capacity: 5L/1L (four units) | 0.5L-10L, customizable
- Working Volume: 30%-75%
- Vessel Material: High borosilicate glass
- Bioreactor Inlets: Flame inoculation port; four-in-one feed inlet(nutrient feed, antifoam, acid, alkali); off-gas port; air inlet
- Air Inlet: Deep aeration design with the air inlet introduced from the upper sidewall of the vessel. The internal air sparger is detachable. The bioreactor is equipped with a U-shaped gas distributor, which ensures uniform gas distribution throughout the vessel with no dead zones. This design enables efficient oxygen transfer, is easy to clean, and fully meets the requirements of high aerobic fermentation processes.
- Off-Gas: Equipped with off-gas condenser and off-gas filter
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Agitation System
- Agitation Type: The bioreactor is equipped with 3-stage, 6-blade impellers, with adjustable installation height. A mechanical defoaming impeller is also provided to effectively control foam formation during operation.
The impeller type and configuration can be customized.
- Agitation Type: The bioreactor is equipped with 3-stage, 6-blade impellers, with adjustable installation height. A mechanical defoaming impeller is also provided to effectively control foam formation during operation.
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Sterile System
- Pre-Filter: Filtration accuracy: 0.3μm; efficiency: 99.99%
- Fine Filter: Filtration accuracy: 0.01μm; efficiency: 99.9999%
- Off-Gas Filter: Filtration accuracy: 0.01μm; efficiency: 99.9999%
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Piping System
- Piping Configuration: Silicone tubing connections for air inlet and off-gas
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Hardware Configuration
- Siemens PLC + 15-inch LCD touch screen
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Control Software
- Dedicated fermentation control software (independent intellectual property)
- Real-time and historical curves; supports 24-hour continuous monitoring and control
- Integrated fermentation process analysis software
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Sterilization
- Off-line sterilization
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Temperature Control
- Display Accuracy: 0.3% FS ±0.1°C
- Control Accuracy: ±0.2°C
- Sensor: WIKA Germany
- Control Function: Dual-direction heating and cooling control; equipped with constant-temperature water tank
- Data Management: Data logging, production curves, report analysis, remote data transmission
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pH
- Display Accuracy: 0.01 pH; measurement range: 0-12 pH
- Sensor: METTLER (Germany) / HAMILTON
- Control Function: Dual-direction acid and alkali addition; digital calibration
- Data Management: Data logging, production curves, report analysis, remote data transmission
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DO
- Display Accuracy: 0.1%; range: 0-200%
- Sensor: METTLER (Germany) / HAMILTON
- Control Function: Cascade control; linked control with agitation speed and aeration
- Data Management: Data logging, production curves, report analysis, remote data transmission
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Agitation Speed
- Speed Range: 0-1000 rpm, customizable
- Data Management: Data logging, production curves, report analysis, remote data transmission
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Gas Flow
- Control Function: Mass flow controller for automatic inlet gas control (maximum aeration up to 2.0 vvm; continuously adjustable according to process requirements)
- Flow Meter: Vögtlin (Switzerland)
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Pressure
- Display Accuracy: 0.01 bar; range: 0-3 bar
- Sensor: BD (Germany)
- Control Function: Digital setpoint control for vessel pressure regulation
- Data Management: Data logging, production curves, report analysis, remote data transmission
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Feeding
- Control Mode: Automatic control
- Programmed Feeding: Time-based programmed feeding with adjustable variable speed
- Batch Feeding: Set feed rate, single batch volume, and feeding cycle
- Programmed Flow Feeding: Pre-set batch volume; multi-batch variable flow feeding based on time profile
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Peristaltic Pumps
- Quantity: Four units: acid addition, alkali addition, antifoam, and feeding
The vessel is made of high-transparency borosilicate glass. The top lid, base, and metal contact parts are made of SUS316L stainless steel with a mirror polish finish (Ra ≤ 0.4μm) to ensure thorough sterilization.
The lid and all process interfaces (feeding, inoculation, exhaust, etc.) feature a comprehensive sanitary design. This effectively minimizes bacterial adhesion and residue, significantly reducing the risk of contamination through physical structure, thereby ensuring process stability and data reproducibility.
Single-Vessel Mode: In-depth development for key projects.
We offer combinations of 2, 3, or 4 glass vessels. One controller can manage up to 4 units simultaneously, supporting multi-dimensional parallel comparative experiments to maximize efficiency and save time.
- Vessel Capacity: 0.5L–10L, customizable
- Number of Vessels: 1–4, customizable
A research institute uses our 1L Quad-unit Glass Fermenter for strain screening. By cultivating in 4 vessels simultaneously under different process conditions, they doubled their output and significantly shortened the R&D cycle.